Test on Genetic Modification and Recombinant DNA
Genetic Modification & Recombinant DNA Explained for Students
Test: Genetic Modification
20 questions
Question 1: Is it true that different types of restriction enzymes are typically used to cut a gene from a donor and to cut the plasmid DNA during the formation of recombinant DNA?
A. Ano
B. Ne
Explanation: To make recombinant DNA, the study materials state that the same restriction enzyme is used to cut the plasmid DNA as was used to cut the gene from the donor. This is necessary to ensure the sticky ends match.
Question 2: The DNA from the donor cell is injected directly into a host cell before being joined with a plasmid.
A. Ano
B. Ne
Explanation: The study materials state that 'DNA from the donor cell is injected into the plasmid' (step 4) and then 'The recombinant plasmid is introduced into a host cell' (step 6). It does not say that donor DNA is injected directly into a host cell before plasmid integration.
Question 3: After DNA ligase joins the sticky ends of the donor cell's DNA with the sticky ends of the plasmid, the resulting recombinant plasmid is then introduced into a host cell.
A. Ano
B. Ne
Explanation: The process of making recombinant DNA concludes with the introduction of the recombinant plasmid into a host cell, as described in the final step of the provided process.
Question 4: The active site of a restriction enzyme is designed to match a broad category of DNA sequences, enabling it to cut DNA at multiple varied locations.
A. Ano
B. Ne
Explanation: Restriction enzymes cut DNA at a specific point because their active site matches a specific DNA sequence, not a broad category allowing cuts at multiple varied locations.
Question 5: Is removing and isolating a plasmid from bacteria a step in the process of making recombinant DNA?
A. Ano
B. Ne
Explanation: The process described for making recombinant DNA includes 'Remove and isolate plasmid from bacteria' as the second step.