Flashcards on Advanced Assisted Reproductive Technologies
Advanced Assisted Reproductive Technologies: A Student's Guide
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In vitro maturation
66 cards
Card 1
Question: What does IVM stand for in assisted reproductive technology?
Answer: In vitro maturation (IVM) of oocytes.
Card 2
Question: Approximately how many IVM births had been estimated by 2015, and how does that compare to births from stimulated cycles?
Answer: About 5,000 IVM births by 2015 versus roughly 5 million births from stimulated cycles.
Card 3
Question: What shift in IVM practice occurred between 1998–2013 according to summarized studies?
Answer: A slow move from 'traditional IVM' to 'pseudo IVM' involving FSH and/or hCG priming.
Card 4
Question: In the Monash 'pseudo IVM' protocol for patients with PCO/PCOS, what FSH priming regimen was used?
Answer: 100–150 IU Gonal‑F given on cycle days 3–6 (FSH priming) with no hCG trigger.
Card 5
Question: When were follicles aspirated in the Monash pseudo‑IVM protocol for PCO/PCOS?
Answer: Aspirate when the leading follicle reached 10–12 mm.
Card 6
Question: Describe the oocyte maturation culture conditions used in Monash pseudo‑IVM before ICSI.
Answer: Mature in G2 Plus medium + 10% patient serum with 0.1 IU/ml FSH and 0.5 IU/ml hCG for 24–26 hours, then perform ICSI.
Card 7
Question: How was the endometrium prepared around oocyte retrieval in the Monash pseudo‑IVM protocol?
Answer: Estradiol (E2) given from the day before oocyte pick‑up (OPU) and progesterone (P) from the day after OPU.
Card 8
Question: In the Monash dataset (Junk et al., Fertil Steril 2012), what overall proportion of collected oocytes reached MII after IVM?
Answer: 588 of 844 oocytes, or 69.7%, reached metaphase II (MII) after IVM.
Card 9
Question: From the same Monash dataset, what percentage of IVM-derived oocytes fertilized normally?
Answer: 420 of 588 MII oocytes fertilized normally, which is 71.4%.
Card 10
Question: What blastocyst formation rate (day 5/6) was reported for embryos from IVM in the Monash dataset?
Answer: 175 blastocysts from 420 normally fertilized oocytes, or 41.7%.