Test on Proteomics: Methods and Applications

Question 1: A MALDI-TOF MS spectrum derived from an in-gel tryptic digest is identified as a peptide map.

A. Ano

B. Ne

Explanation: The study materials explicitly label a 'MALDI-TOF MS spectrum of in-gel tryptic digest' as a 'peptide map', indicating that the spectrum itself represents the set of masses of these digested peptides.

Question 2: According to the study materials, which of the following is a key step in identifying microorganisms using MALDI-MS profiling?

A. Analyzing specific protein digestion fragments through MS/MS data.

B. Comparing the measured MALDI-MS profile of the microorganism with a database of known MALDI-MS profiles.

C. Determining the unknown sequence of peptides via de novo sequencing.

D. Separating intact proteins using 2-D gel electrophoresis before MS analysis.

Explanation: The study materials state that microorganism identification by MALDI-MS profiling involves 'comparison of measured profile with database profile' and 'database of MALDI-MS profiles'. This method relies on comparing the obtained spectral profile (ranging from 3-20 kDa peptides/proteins) against a known database for identification. The other options describe methods for protein identification or characterization, but not the specific microorganism identification by MALDI-MS profiling as described in the initial sections of the study materials.

Question 3: The LC-MS/MS PRM tryptic digestion method used for amelogenin isoform analysis for sex determination is a non-destructive approach.

A. Ano

B. Ne

Explanation: The study materials explicitly state that the LC-MS/MS PRM tryptic digestion method used for amelogenin isoform analysis is a 'destructive method' for sex determination.

Question 4: Hundreds of thousands of proteins were identified in the dental calculus analysis of Gregor Johann Mendel.

A. Ano

B. Ne

Explanation: The analysis of dental calculus of Gregor Johann Mendel identified 469,289 proteins (with >2 peptides).

Question 5: Which of the following statements about amelogenin isoforms and their use in sex determination, according to the provided study materials, is correct?

A. The presence of both AMELX and AMELY indicates a female individual.

B. AMELY typically has a significantly higher intensity than AMELX in destructive LC-MS/MS PRM methods.

C. AMELX alone is sufficient for female determination, or for identifying a male if AMELY is below detection limits.

D. The non-destructive method for amelogenin analysis involves mechanical enamel grinding and SDT buffer extraction.

Explanation: According to the study materials, the presence of AMELX alone indicates a female individual, or a male where AMELY is below the detection limit. The presence of both AMELX and AMELY indicates a male. AMELY typically shows approximately 10x lower intensity than AMELX in destructive methods. Mechanical enamel grinding and SDT buffer extraction are part of the destructive method, not the non-destructive one described by Steward et al.