Flashcards on Plant Collection for Ethnobotany and Ethnopharmacology
Plant Collection for Ethnobotany and Ethnopharmacology Guide
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Cryopreservation and Plant Sample Processing
11 cards
Card 1
Question: What is cryopreservation?
Answer: A process where cells, tissues and organs (including dormant buds and pollen) susceptible to damage from chemical reactivity (e.g., enzymes) are prese
Card 2
Question: What temperature range is given for cryopreservation in the content?
Answer: Between −136 and −196 °C.
Card 3
Question: What types of plant material are mentioned as uses for cryopreservation?
Answer: Vegetative material of clonally (vegetatively) propagated species and non-orthodox (recalcitrant) seeds.
Card 4
Question: Name three types of equipment or methods listed under processed research materials for plant sample processing.
Answer: Drying ovens/rooms; Open air; Microwave & infrared devices.
Card 5
Question: What is lyophilization (freeze-drying) in the context of plant sample processing?
Answer: Freezing and reducing the pressure to allow frozen water in the material to sublimate, typically from −50 to −80 °C.
Card 6
Question: Why are low drying temperatures (30–50 °C) recommended for processing plant materials?
Answer: To protect sensitive active ingredients.
Card 7
Question: What fuels are mentioned as acceptable for drying at temperatures below 60 °C?
Answer: Butane, propane or natural gas.
Card 8
Question: How should plant material be placed for drying to ensure proper processing?
Answer: Shaded from direct sunlight, placed in thin layers on drying frames, wire-screened rooms or buildings.
Card 9
Question: What are the advantages of lyophilization mentioned in the content?
Answer: Shorter processing time and uniform material temperature.
Card 10
Question: Which plant derivatives processing methods are listed?
Answer: Hydro/steam distillation and extraction & evaporation.