Flashcards on Introduction to Chromatography
Introduction to Chromatography: Principles & Applications
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Chromatography
15 cards
Card 1
Question: What is chromatography in simple terms?
Answer: A way to separate substances dissolved in a mixture so each substance can be identified and analysed.
Card 2
Question: What is paper chromatography used for?
Answer: To find out which colours are mixed together in paints, dyes and inks using special chromatography paper and a solvent.
Card 3
Question: How do you set up a paper chromatography experiment?
Answer: Put a concentrated dot of the mixture near the bottom of chromatography paper, place the paper with its bottom in a solvent, and let the solvent carry
Card 4
Question: What is a chromatogram?
Answer: The pattern formed on the paper (or displayed as a graph) that shows separated substances after chromatography.
Card 5
Question: Why do different substances separate during chromatography?
Answer: Because the solvent carries each substance at different speeds, so they move apart from each other.
Card 6
Question: In machine chromatography shown as a graph, what does each peak mean?
Answer: Each peak represents a different substance in the mixture.
Card 7
Question: In the machine chromatography graph, what does the height of a peak show?
Answer: How much of that substance was in the original sample (its concentration).
Card 8
Question: How can colourless substances be seen on a chromatogram?
Answer: The chromatogram can be treated to make substances coloured or ultraviolet light can make them glow so they become visible.
Card 9
Question: Give two real-world uses of chromatography mentioned in the text.
Answer: Testing drinking water for substances and testing blood or urine for banned drugs (athletic tournaments).
Card 10
Question: How is chromatography used in forensic analysis?
Answer: To identify specific mixtures, such as colours in a lipstick or in a black ink at a crime scene.