Question 1

What is Enzyme activity is the rate of substrate converted to product; units?

Accepted Answer

U and kat with 1 \mu kat = 60 U

Question 2

What is Michaelis?

Accepted Answer

Menten equation: $v_0 = \frac{V_{\max}[S]}{K_m + [S]}$ where $K_m$ is [S] at $v_0=	frac{1}{2}V_{\max}$

Question 3

What is Lineweaver?

Accepted Answer

Burk: $\frac{1}{v_0} = \frac{K_m}{V_{\max}}\frac{1}{[S]} + \frac{1}{V_{\max}}$; y-intercept $=1/V_{\max}$

Question 4

What is Non?

Accepted Answer

competitive inhibitors reduce $V_{\max}$ without changing $K_m$ for pure non-competitive cases

Question 5

What is Suicide inhibitors are mechanism?

Accepted Answer

based irreversible inhibitors used in medicine (e.g. 5-FU, allopurinol)

Question 6

What is Phosphorylation/dephosphorylation regulate enzyme activity rapidly; example?

Accepted Answer

glycogen phosphorylase

Enzyme Kinetics and Regulation

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